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CENTRAL DOGMA
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Reading
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Links
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Sept
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7
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What is molecular biology? Cellular
components
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Chap. 1
"Are Viruses Alive" & Quiz
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9
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Why is DNA a double helix with only four
bases? - DNA structure + recombination
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p. 111-120
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Link
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*
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12
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PyMol tutorial – an introduction to protein
structure
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Chap. 3, p.286-296
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14
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Transcription factors and other DNA binding
proteins
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Chap. 3, p.286-296
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Link
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16
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How do we make DNA without making mistakes?
- DNA replication/repair
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139-145, 1139-1146
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Link
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19
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Copying DNA in the lab: Polymerase Chain
Reaction and Sequencing
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184-190
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Link
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21
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Transcription
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271-276, 323-336
"The Alternative Genome" & Quiz
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Link
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23
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Lac operon - Transcription
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26
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Learn how to speak nucleic and amino acid in
one day! - Translation
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127-139
"Evolution Encoded" & Quiz
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Link
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28
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Review and Overview
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30
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EXAM 1 - 70 points
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MOLECULAR SWITCHES IN THE LIVER
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Link
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Oct
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3
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Role of DNA and chromatin structure
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215-237
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5
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Methylation and acetylation of histones and
DNA
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247-257
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7
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Proteins regulating transcription
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276-286, 296-313
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10
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Post transcriptional regulation of gene
expression
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347-358
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12
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Proteins as molecular switches
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Chap. 15
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14
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Signal transduction and receptors
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Chap. 16
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17
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Gene
chips to measure transcription
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19
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Review and overview
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21
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EXAM 2 - 100 points
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CELLULAR POKER: HOLD, RAISE OR FOLD
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24
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The game is "cell cycle"
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Chap. 20
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Link
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26
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Player 1: Apoptosis - folds
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936-944
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Link
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28
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Player 2: Cancer - doubles and doubles again
and …
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Chap. 25
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Link
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31
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Player 3: Developmental biology - changes
the game
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969-984
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Link
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RECOMBINANT HUMAN tPA AS A DRUG
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Nov
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2
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Overview: Identification of the tissue
producing tPA, cDNA and in vitro translation
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176-184, 188-198
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Link
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4
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Basic cloning techniques, restriction
enzymes, plasmids, ligase
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7
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Production of a cDNA library containing tPA.
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*
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9
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Screening libraries, DNA sequencing, and
sequence analysis
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11
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Cloning and expression of the tPA cDNA in E.
coli.
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14
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Expression of tPA in yeast and mammalian
cells Making a good thing better: In vitro
mutagenesis
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16
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HAND OUT EXAM 3 (take home)
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18
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Green mice - introducing genes into animals
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204-211
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21
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Gene therapy, transgenic plants and animals
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23
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Genomics EXAM
3 DUE - 130 points
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25
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THANKSGIVING
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28
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Genomics
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243-247
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Link
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30
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Mapping the BRCA1 gene
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198-204
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Link
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Dec
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2
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Identification of proteins interacting with
BRCA1
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5
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BRCA1 KO mice
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7
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Role of BRCA1 in DNA repair
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9
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Role of BRCA1 in DNA repair
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12
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Regulation of gene expression by BRCA1 -
microarrays
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192-195
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14
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Comparative genomics of BRCA1 and Molecular
Evolution
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* indicates a class held in a computer lab,
7 Wing
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