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CENTRAL DOGMA
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Reading
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Links
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Sept
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9
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What is molecular biology?
Cellular components
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Chap. 1
"Are Viruses
Alive" & Quiz
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11
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Why is DNA a double helix with only four
bases? - DNA structure + recombination
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p. 111-120
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Link
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*
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14
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PyMol tutorial – an introduction to protein structure |
Chap. 3, p.286-296 |
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16 |
Transcription factors and other DNA binding proteins |
Chap. 3, p.286-296 |
Link |
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18
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How do we make DNA without making mistakes?
- DNA replication/repair
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139-145, 1139-1146 |
Link
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21
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Copying DNA in the lab:
Polymerase Chain Reaction and Sequencing
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184-190
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Link |
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23
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Lac operon - Transcription
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271-276,
323-336 "The Alternative
Genome" & Quiz
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Link |
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25
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Learn how to speak nucleic and amino acid
in one day! - Translation
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127-139 "Evolution
Encoded" & Quiz
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Link |
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28
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Review and Overview
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30
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EXAM 1 - 70 points
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MOLECULAR SWITCHES IN THE LIVER
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All on one page |
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Oct
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2
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Role of DNA and chromatin structure
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215-237 |
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5
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Methylation and acetylation of
histones and DNA |
247-257 |
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7
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Proteins regulating transcription
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276-286, 296-313 |
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9
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Post
transcriptional regulation of gene expression
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347-358 |
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12
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Proteins
as molecular switches
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Chap. 15 |
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14
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Signal transduction and receptors
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Chap. 16 |
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16 |
Gene
chips to measure transcription
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19
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Review and overview
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21
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EXAM 2 - 100 points
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CELLULAR POKER: HOLD, RAISE OR FOLD
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23
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The game is "cell cycle"
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Chap. 20 |
Link |
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26
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Player 1:
Apoptosis - folds
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936-944
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Link |
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28 |
Player
2:
Cancer - doubles and doubles again and …
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Chap. 25 |
Link |
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30
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Player
3: Developmental biology - changes the game
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969-984 |
Link |
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RECOMBINANT HUMAN tPA AS A DRUG
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Nov
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2
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Overview:
Identification of the tissue producing tPA, cDNA and in
vitro translation
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176-184, 188-198
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All
on one page |
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4
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Basic cloning techniques, restriction
enzymes, plasmids, ligase
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6
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Production of a cDNA library containing tPA.
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9
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Screening
libraries, DNA sequencing, and sequence analysis
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*
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11
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Cloning and expression of the tPA cDNA in
E. coli.
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13
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Expression of tPA in yeast and mammalian
cells
Making a good thing better:
In vitro
mutagenesis
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HAND OUT EXAM 3 (take home)
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GREEN MICE AND HAM, SAM I AM
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16
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No Class
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18
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Green mice - introducing genes into animals
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204-211 |
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20
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Gene therapy, transgenic plants and
animals
EXAM 3 DUE - 130
points
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100,000 GENES ON THE WALL…
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23
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So much DNA, so little time - genomics
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243-247 |
Link
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25 |
No Class |
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27 |
THANKSGIVING
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30
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Mapping the BRCA1 gene
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198-204 |
All
on one page |
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Dec
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2
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Identification of proteins interacting with
BRCA1
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4 |
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7
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BRCA1 KO mice
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9
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Role of BRCA1 in DNA repair
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11 |
Role of BRCA1 in DNA repair
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14
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Regulation of gene expression by BRCA1 -
microarrays
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192-195
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16
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Comparative genomics of BRCA1 and Molecular
Evolution
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* indicates a class held in a computer lab,
7 Wing
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