Assignment #1, v. 1.1A

Please paste this assignment into a word processor program and place your answers below each of the questions.

Using the GenBank accession number V00519 as a starting point, answer the following questions:

1.What GenBank sequence does the accession number specify?  What does this sequence encode?

2.How many bases of sequence are in the file?  How many total in GenBank (as of this date)?

3.Use Medline to answer the following questions:

a. How many amino acids are in the "precursor region?"

b. In what organism has this protein been expressed?

b. To what bacterial gene was the cDNA fused?

4.Several groups have reported the sequence used to create this file.  At what  positions do their sequences differ?  What are the specific differences?

5.Retrieve the file HSGROW2.  From what tissue was this sequence derived?

6.How many bases are in the primary transcript?

7.How many introns are removed as the primary transcript is processed?

8.At what specific bases does the start codon appear?  At what specific base does the stop codon appear?  Which of the three stop codons is used? 

9.What is the accession number for the protein sequence corresponding to the nucleic acid sequence specified above?  Retrieve this file to answer the next series of questions.

10.How many amino acids are there in this protein?  What are the first five amino acids beginning at position 141 (Please use the entire names of these amino acids - no abbreviations)?

11.Specify three genetic disorders that can result if this protein does not function properly. (HINT: Search OMIM through Entrez with 'hgh' as a query).

12.Where does this gene map in the human genome? (HINT: Use the Entrez LocusLink MapViewer to access this information using GH1 as a query).  What two markers is this gene between?  What is encoded by these flanking markers?