Clostridium perfringens

For Clostridium perfringes to fully grow there are certain growth requirements that are needed in order for the bacterium to reach its full growth potential. These include situations where it can be in an anaerobic living condition. Be grown at 37°C, which is equivalent to the temperature in the human body. And must also have basic sugars such as glucose for  glycolysis or glycogen for glycogen metabolism. But this bacterium is lacking some very important amino acids and vitamins and they are:

9 amino acids                           
-arginine                                          
-glutamic acid
-histidine
-isoleucine
-leucine
-methionine
-phenylalanine
-threonine
-tryptophane
-tyrosine
-valine

and 2 vitamins: (Sebald et al)
-panthothenic acid
-pyridoxamine

Experimentally speaking when Sebald et al discovered these 11 important growth factors for C. perfringens they discovered that the bacterium doesn't have the machinery to create any of these core nutrients. Which means that the bacteria is dependent on its environment in order to get these nutrients for growth.

In order to grow this bacterium in a laboratory it is essential that the bacteria is given all these requirements. If all of these nutrients are not present there will be no substantial growth discovered on the media being used. An example of a plate with all the proper nutrients is the egg agar image displayed here which when taking an egg white all of the nutrients that were present in the egg are now available for bacterial use.