Assignment #1, v. 1.1D

Due in class: January 24^th 2001

Please paste this assignment into a word processor program and place your answers below each of the questions.

Using the GenBank accession number NM_000207 as a starting point, answer the following questions:

1.What GenBank sequence does the accession number specify?  What does this sequence encode?

2.How many bases of sequence are in the file?  How many total in GenBank (as of this date)?

3.Use Medline to answer the following questions:

a. What are the names of the authors that published the first sequence of the

         gene?

b. How many introns in this gene?  Where are they located?

c. When the gene was expressed in E. coli what was the protein fused to?

d. From what country were the scientists who made this fusion?

4.How many bases are in the primary transcript?

5.How many "g" residues appear in the reported sequence?  Calculate the G+C ratio of this sequence.

6.At what specific bases does the start codon appear?  At what specific base does the stop codon appear?  Which of the three stop codons is used? 

7.What is the accession number for the protein sequence corresponding to the nucleic acid sequence specified above?  Retrieve this file to answer the next series of questions.

8.How many amino acids are there in this protein?  What are the first five amino acids beginning at position 71 (Please use the entire names of these amino acids - no abbreviations)?

9.How is the translation product processed into the final product?  What does the final product bind to?  What happens in our bodies after the product binds?

10.What specific cells in our bodies produce the protein encoded by this gene? (HINT: Search OMIM through Entrez with 'INS' as a query).

11.Where does this gene map in the human genome? What two markers is this gene between?  What is encoded by these flanking markers?