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1. First identify the start codon in the open reading frame. Then break the sequence into 3 base pair codons until you reach a stop codon. Finally translate each codon into its corresponding amino acid. GGGATCGATGCCCCTTAAAGAGTTTACATATTGCTGGAGGCGTTAACCCCGGA DNA AUG CCC CUU AAA GAG UUU ACA UAU UGC UGG AGG CGU UAA mRNA Met Pro Leu Lys Glu Phe Thr Tyr Cys Trp Arg Arg Stop Protein
2. First write the complementary strand of DNA. Next look for the longest possible ORF in both strands, reading each in a 5´ to 3´ direction. In this instance the longest open reading frame is in the reverse orientation (strand 2). Strand 2 will be the sense strand and Strand 1 the antisense strand Strand 1 5' TCAATGTAACGCGCTACCCGGAGCTCTGGGCCCAAATTTCATCCACT 3' Strand 2 3´ AGTTACATTGCGCGATGGGCCTCGAGACCCGGGTTTAAAGTAGGTGA 5´ 1 S M * R A T R S S G P K F H P 2 Q C N A L P G A L G P N F I H 3 N V T R Y P E L W A Q I S S T 4 * H L A S G P A R P G F K M W 5 E I Y R A V R L E P G L N * G 6 K L T V R * G S S Q A W I E D
mRNA 5´ AGUGGAUGAAAUUUGGGCCCAGAGCUCCGGGUAGCGCGUUACAUUGA 3´ Protein N- Met Lys Phe Gly Pro Arg Ala Pro Gly Ser Ala Leu His -C |
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